EEF1E1 Protein (AA 1-174) (His tag)

Catalog No. ABIN667425

Product Details

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Protein Type: Recombinant

Protein Characteristics: AA 1-174

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EEF1E1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: EEF1E1, 1-174aa, Human, His tag, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Storage: 4 °C

Target Details

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: EEF1E1 (EEF1E1 Products)

Synonyms: p18 Protein, AIMP3 Protein, P18 Protein, 1110003A02Rik Protein, eukaryotic translation elongation factor 1 epsilon 1 Protein, eukaryotic translation elongation factor 1 epsilon 1 L homeolog Protein, EEF1E1 Protein, eef1e1.L Protein, eef1e1 Protein, Eef1e1 Protein

Background: EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18. NCBI no.: NP_004271

Molecular Weight: 21.9 kDa (194aa) confirmed by MALDI-TOF

Pathways: Positive Regulation of Response to DNA Damage Stimulus