POU Class 2 Associating Factor 1 (POU2AF1) (AA 1-256) protein (His tag)

Details for Product No. ABIN667494, Supplier: Log in to see
Protein Name
  • BOB1
  • OBF-1
  • OBF1
  • OCAB
  • BOB-1
  • BOB.1
  • Bob-1
  • Bob1
  • OBF.1
  • OCA-B
  • POU class 2 associating factor 1
  • POU domain, class 2, associating factor 1
  • POU2AF1
  • Pou2af1
Protein Characteristics
AA 1-256
8
5
1
1
Origin
Human
8
1
1
Source
Escherichia coli (E. coli)
6
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This POU2AF1 protein is labelled with His tag.
Application
SDS-PAGE (SDS)
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Characteristics POU2AF1, 1-256aa, Human, His tag, E.coli
Purity > 85 % by SDS - PAGE
Background POU2AF1, also known as BOB1, is a lymphocyte specific transcription coactivator protein. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, POU2AF1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. POU2AF1 is expressed at highest levels in spleen and peripheral blood leukocytes. Recombinant human POU2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: POU domain class 2-associating factor 1, BOB1, OBF-1, OBF1, OCAB. NCBI no.: NP_006226
Molecular Weight 29.6 kDa (276aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml (determined by Bradford assay)
Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
Storage 4 °C
Supplier Images
SDS-PAGE (SDS) image for POU Class 2 Associating Factor 1 (POU2AF1) (AA 1-256) protein (His tag) (ABIN667494) POU Class 2 Associating Factor 1 (POU2AF1) (AA 1-256) protein (His tag)
Background publications Luo, Roeder: "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4115-24, 1995 (PubMed).

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