POLB Protein (AA 1-335) (His tag)
-
- Target See all POLB Proteins
- POLB (Polymerase (DNA Directed), beta (POLB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-335
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- POLB, 1-335aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLB Protein
-
Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His tag)
POLB Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 224-333) protein (GST tag)POLB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (Strep Tag)Crystallography grade POLB Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), beta (POLB) (Active) proteinPOLB Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % pure by SDS-PAGE analysis Func Active
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
- Storage
- 4 °C
-
- Target
- POLB (Polymerase (DNA Directed), beta (POLB))
- Alternative Name
- POLB (POLB Products)
- Synonyms
- wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein
- Background
- DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: DNA polymerase beta, DNA directed DNA polymerase beta, DNA pol beta, DNA polymerase beta, DNA polymerase beta subunit, MGC125976, Pol B, Pol beta, PolB, Polymerase (DNA directed) beta. NCBI no.: NP_002681
- Molecular Weight
- 40.3 kDa (355aa), confirmed by MALDI-TOF
- Pathways
- DNA Damage Repair
-
