UBA3 Protein (AA 1-463) (His tag)
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- Target See all UBA3 Proteins
- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-463
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBA3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- UBA3, 1-463aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product UBA3 Protein
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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 1-463) protein (GST tag)
UBA3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 1-463) protein (His tag)UBA3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 2-463) protein (His tag)Crystallography grade UBA3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBA3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBA3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
- Storage
- 4 °C
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- Target
- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
- Alternative Name
- UBA3 (UBA3 Products)
- Synonyms
- UBE1C Protein, hUBA3 Protein, Ube1c Protein, ube1c Protein, wu:fb75e04 Protein, wu:fc37b11 Protein, zgc:55528 Protein, A830034N06Rik Protein, AI256736 Protein, AI848246 Protein, AW546539 Protein, ubiquitin like modifier activating enzyme 3 Protein, ubiquitin-like modifier activating enzyme 3 Protein, ubiquitin like modifier activating enzyme 3 S homeolog Protein, UBA3 Protein, Uba3 Protein, uba3 Protein, uba3.S Protein
- Background
- UBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Recombinant human UBA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: UBE1C. NCBI no.: AAH22853
- Molecular Weight
- 54.4 kDa (487aa)
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