PKLR Protein (AA 47-574) (His tag)
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- Target See all PKLR Proteins
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 47-574
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PKLR protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- PKLR, 47-574aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PKLR Protein
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Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (GST tag)
PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 485-574) protein (GST tag)PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (Strep Tag)Crystallography grade PKLR Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
- Storage
- 4 °C
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- Target
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
- Alternative Name
- PKLR (PKLR Products)
- Synonyms
- PK1 Protein, PKL Protein, PKR Protein, PKRL Protein, RPK Protein, Pklg Protein, wu:fd15e01 Protein, wu:fi37e08 Protein, pk1 Protein, PKLR Protein, Pk-1 Protein, Pk1 Protein, R-PK Protein, pklr Protein, pyruvate kinase L/R Protein, pyruvate kinase, liver and RBC Protein, pyruvate kinase, liver and RBC L homeolog Protein, pyruvate kinase liver and red blood cell Protein, pyruvate kinase PKLR-like Protein, PKLR Protein, Pklr Protein, pklr Protein, pklr.L Protein, LOC100621940 Protein
- Background
- PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK. NCBI no.: NP_000289
- Molecular Weight
- 59.2 kDa (549aa), confirmed by MALDI-TOF
- Pathways
- Ribonucleoside Biosynthetic Process
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