MYL5 Protein (AA 1-173) (His tag)
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- Target See all MYL5 Proteins
- MYL5 (Myosin, Light Chain 5, Regulatory (MYL5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-173
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MYL5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- MYL5, 1-173aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product MYL5 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
- Storage
- 4 °C
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- Target
- MYL5 (Myosin, Light Chain 5, Regulatory (MYL5))
- Alternative Name
- MYL5 (MYL5 Products)
- Synonyms
- MYLC2 Protein, MyLC-2 Protein, myosin light chain 5 Protein, MYL5 Protein
- Background
- Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Myosin light chain 5 Isoform1,. NCBI no.: NP_002468
- Molecular Weight
- 22.1 kDa(197aa), confirmed by MALDI-TOF
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