EIF3S1 Protein (AA 70-258) (His tag)

Catalog No. ABIN667972

Product Details

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Protein Type: Recombinant

Protein Characteristics: AA 70-258

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EIF3S1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: EIF3J, 70-258aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl

Storage: 4 °C

Target Details

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Alternative Name: EIF3J (EIF3S1 Products)

Synonyms: GB12381 Protein, An18g06260 Protein, AO090003000054 Protein, EIF3S1 Protein, eIF3j Protein, eIF3-alpha Protein, eIF3-p35 Protein, Eif3s1 Protein, eif3s1 Protein, 1810016I04Rik Protein, 2700079K05Rik Protein, AA409117 Protein, AA409446 Protein, C78575 Protein, Eif3j Protein, Eif3j-1 Protein, Eif3j-2 Protein, Eif3j2 Protein, eIF-3-alpha-A Protein, eIF-3-alpha-B Protein, eIF3j-A Protein, eIF3j-B Protein, eukaryotic translation initiation factor 3 subunit J Protein, eukaryotic translation initiation factor 3, subunit J Protein, Eukaryotic translation initiation factor 3 subunit J Protein, eukaryotic translation initiation factor 3 subunit 1 Protein, eukaryotic translation initiation factor 3 subunit J S homeolog Protein, eukaryotic translation initiation factor 3, subunit J1 Protein, LOC409796 Protein, ANI_1_830164 Protein, AOR_1_94154 Protein, Eif3j Protein, EIF3J Protein, eif3j Protein, LOC733089 Protein, eif3j.S Protein, Eif3j1 Protein

Background: EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA. Recombinant human EIF3J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Eukaryotic translation initiation factor 3, subunit J, eIF3-alpha, eIF3-p35, EIF3S1. NCBI no.: NP_003749

Molecular Weight: 24.0kDa (210aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Pathways: Ribonucleoprotein Complex Subunit Organization