PHOSPHO2 Protein (AA 1-241) (His tag)
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- Target See all PHOSPHO2 Proteins
- PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-241
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PHOSPHO2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- PHOSPHO2, 1-241aa, Human, His tag, E.coli
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product PHOSPHO2 Protein
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Phosphatase, Orphan 2 (PHOSPHO2) (AA 1-241) protein (His tag)
PHOSPHO2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphatase, Orphan 2 (PHOSPHO2) (AA 1-241) protein (Strep Tag)Crystallography grade PHOSPHO2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Phosphatase, Orphan 2 (PHOSPHO2) (AA 1-241) protein (Strep Tag)Crystallography grade PHOSPHO2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
- Storage
- 4 °C
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- Target
- PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))
- Alternative Name
- PHOSPHO2 (PHOSPHO2 Products)
- Synonyms
- zgc:172082 Protein, 1700048E23Rik Protein, AI661517 Protein, AU021728 Protein, AV006103 Protein, Phos2 Protein, phosphatase, orphan 2 Protein, phosphatase, orphan 2 L homeolog Protein, PHOSPHO2 Protein, phospho2 Protein, phospho2.L Protein, Phospho2 Protein
- Background
- Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase(HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2. NCBI no.: NP_001008489
- Molecular Weight
- 30.3 kDa (265aa), confirmed by MALDI-TOF
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