INHBB Protein (His tag)

Catalog No. ABIN809201

Product Details

Target: INHBB (Inhibin, beta B (INHBB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Purification tag / Conjugate: This INHBB protein is labelled with His tag.

Application: Western Blotting (WB), Cell Culture (CC)

Characteristics: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin B.
Molecular formula: C615H910N178O177S12.
Isoelectric Point: 6,4.
Extinction coefficient: E 0.1 % (1g/L) = 1.56 (A 280 nm).
This product contains no animal-derived components or impurities. Produced by transient expression of Activin B in non-transgenic plants.

Purification: Recombinant human Activin B contains a His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Purity: > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

Biological Activity: The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. Less than 5 ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.

Buffer: Tris HCl 0.05 M buffer at pH 7.4

Storage: 4 °C

Target Details

Target: INHBB (Inhibin, beta B (INHBB))

Alternative Name: INHBB (INHBB Products)

Synonyms: actbb Protein, actbetaB Protein, inhibin Protein, inhibin, beta B Protein, inhibin beta B L homeolog Protein, inhibin beta B subunit Protein, inhibin beta B Protein, inhibin beta-B Protein, inhibin beta B chain Protein, inhbb Protein, inhbb.L Protein, INHBB Protein, Inhbb Protein, LOC100719829 Protein

Background: Synonyms: Activin beta-B chain, Inhibin beta B chain precursor, Inhibin, beta-2
Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGF-beta family. Mature Activin B has two chains of 123 amino acids residues (betaB-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins/Activins are protein that are formed by the dimerization of two subunits, i. e. an alpha (alpha) with either beta A (betaA) - Inhibin A or beta B (betaB) - Inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers called Activin: Activin A (betaA -betaA), Activin B (betaB -betaB) and Activin AB (betaA -betaB). The Activin gene family comprises the additional, but poorly characterized members’ Activin betaC (betaC), beta D (betaD) and beta E (betaE). As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B.

Molecular Weight: 14 kDa

Gene ID: 3625, 147390

Pathways: Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion