PSPH Protein (AA 1-382) (His tag)
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- Target See all PSPH Proteins
- PSPH (Phosphoserine Phosphatase (PSPH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-382
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PSPH protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE
- Characteristics
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Purified recombinant Human Phosphoserine phosphatase protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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Phosphoserine Phosphatase (PSPH) (AA 1-225) protein (GST tag)
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Phosphoserine Phosphatase (PSPH) (AA 1-100) protein (GST tag)PSPH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoserine Phosphatase (PSPH) (AA 1-225) protein (Strep Tag)Crystallography grade PSPH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoserine Phosphatase (PSPH) protein (Myc-DYKDDDDK Tag)PSPH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Phosphoserine phosphatase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 10 mM Tris-HCl, pH 7.5, containing 50 mM NaCl, 0 mM DTT, 0 mM MnCl, and 20 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- PSPH (Phosphoserine Phosphatase (PSPH))
- Alternative Name
- Phosphoserine phosphatase (PSPH Products)
- Synonyms
- PSP Protein, PSPHD Protein, AI480570 Protein, PSPase Protein, PSPH Protein, psph Protein, MGC78828 Protein, zgc:112414 Protein, DDBDRAFT_0183847 Protein, DDBDRAFT_0230054 Protein, DDB_0183847 Protein, DDB_0230054 Protein, phosphoserine phosphatase Protein, phosphoserine phosphatase L homeolog Protein, PSP; O-phosphoserine phosphohydrolase; PSPase Protein, phosphoserine phosphatase SerB Protein, BPI fold containing family A member 2 Protein, PSPH Protein, Psph Protein, psph.L Protein, psph Protein, serB Protein, serB-1 Protein, LOC5568588 Protein, PSP Protein, Svir_29300 Protein, MMAH_RS03435 Protein, BPIFA2 Protein
- Background
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Protein phosphatase 2C (PP2Calpha) is a Mn ?+ - or Mg ?+ -dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Calpha(AA 1-382) was cloned into an E. coli expression vector. PP2Calpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
Alternative Names: EC 3.1.3.3 protein, PSPase protein, PSP protein, L 3 phosphoserine phosphatase protein, , Phosphoserine phosphatase deficiency protein, included protein, Phosphoserine phosphatase Human protein, L-3-phosphoserine phosphatase protein, Phosphoserine phosphatase protein, O phosphoserine phosphohydrolase protein, O-phosphoserine phosphohydrolase protein, PSPH protein - Molecular Weight
- 25 kDa (225 AA)
- Pathways
- Warburg Effect
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