ENO2/NSE Protein (AA 1-434)

Catalog No. ABIN934970

Product Details

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-434

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

Characteristics: Purified recombinant Human NSE protein
Expression System: E.coli
Bioactivity: Specific activity: > 8 units/mL. One unit will convert 1.0 m of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25C
Molecular weight on SDS-PAGE will appear higher.

Purity: > 95 % pure

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: NSE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris, pH 7.5, containing 0.1 M KCl, and 5 mM MgSO4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE (ENO2 Products)

Synonyms: ENO2 Protein, DKFZp459B1817 Protein, NSE Protein, AI837106 Protein, D6Ertd375e Protein, Eno-2 Protein, RNEN3 Protein, eno3 Protein, wu:fc09h05 Protein, zgc:92418 Protein, enolase 2 Protein, enolase 2 (gamma, neuronal) Protein, enolase 2, gamma neuronal Protein, enolase 2, gamma, neuronal Protein, ENO2 Protein, Eno2 Protein, eno2 Protein

Background: Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: ENO2 protein, Enolase 2 gamma neuronal protein, neuronal) protein, Neuron specific enolase protein, Neuron specific gamma enolase protein, NSE protein, Gamma enolase protein, 2 phospho D glycerate hydrolyase protein, ENOG protein, Neuron-Specific Enolase protein, Eno 2 protein, Neurone specific enolase., Enolase 2 (gamma protein, Neural enolase protein, Enolase2 protein

Molecular Weight: 47 kDa ( 434 AA)