STUB1 Protein (AA 1-303)

Catalog No. ABIN935009

Product Details

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-303

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY

Characteristics: Purified recombinant Human CHIP protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Purity: > 90 % pure

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: CHIP protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Alternative Name: CHIP (STUB1 Products)

Synonyms: CHIP Protein, HSPABP2 Protein, NY-CO-7 Protein, SDCCAG7 Protein, UBOX1 Protein, 0610033N24Rik Protein, 2210017D18Rik Protein, 2310040B03Rik Protein, AW046544 Protein, Chip Protein, wu:fc22f04 Protein, zgc:56076 Protein, STUB1 Protein, chip Protein, ATCHIP Protein, CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN Protein, carboxyl terminus of HSC70-interacting protein Protein, STIP1 homology and U-box containing protein 1 Protein, STIP1 homology and U-Box containing protein 1 Protein, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase L homeolog Protein, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase Protein, STIP1 homology and U box-containing protein 1 Protein, carboxyl terminus of HSC70-interacting protein Protein, STUB1 Protein, Stub1 Protein, stub1 Protein, stub1.L Protein, LOC100282870 Protein, CHIP Protein

Background: CHIP, also known as STUB1, is a cytoplasmic protein whose amino acid sequence is highly conserved across species. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, whereas its U-box domain contains its E3 ubiquitin ligase activity. Its interaction with these molecular chaperones results in client substrate ubiquitylation and degradation by the proteasome. Thus, CHIP acts to tilt the folding-refolding machinery toward the degradative pathway, and it serves as a link between the two. Recombinant human CHIP protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: NY CO 7 protein, STUB 1 protein, CHIP protein, HSPABP2 protein, STIP1 homology and U Box containing protein 1., E3 ubiquitin protein ligase CHIP protein, STUB1 protein, UBOX1 protein, Carboxy terminus of HSP70 interacting protein protein NY CO 7 protein, SDCCAG7 protein, UBOX 1 protein, NY-CO-7 protein, Carboxy terminus of Hsp70p interacting protein protein, Serologically defined colon cancer protein 7 protein, CLL associated protein KW 8 protein, Heat shock protein A binding protein 2 (c terminal) protein

Molecular Weight: 34.8 kDa (303 AA)

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation