(2 references)-
- Target
- PsbA
- Protein Type
- Recombinant
- Host
- Please inquire
- Application
- Western Blotting (WB)
- Brand
- SERVA®
- Purification
- affinity purified
-
- Application Notes
- Standard curve : 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2µg of chlorophyll will give a PsbA signalin this range. positive control : a 2µl load per well is optimal for most chemiluminescent detectionsystems. Non-disulphie dependent dimers and complexes can be also detected usingstandard western blot methods with more sensitive detection reagents as ECLAdvance or West Pico when loading per well more standard than recommended.They have not been included in the standard calibration. Additional Information: global antibodies are raised against highly conserved amino acid sequences inthe PsbA protein. The PsbA protein standard can therefore be used incombination with global anti-PsbA antibodies to quantitate PsbA from a widerange of species. Quantitative western blot : detailed method description.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- 0.25 pmol/µL
- Buffer
- Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/mL PefaBloc protease inhibitor (Roche), 50 mM DTT. This standard is ready-to-load and does not require any additions or heating.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
-
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: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).
: "Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study." in: The ISME journal, Vol. 2, Issue 9, pp. 937-53, (2008) (PubMed).
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: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).
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- Target
- PsbA
- Background
- The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.This is a recombinant protein standard, source: Synechocystis PCC 6803.
- Molecular Weight
- 41.5 kDa (larger than native protein of 38 kDa due to the addition of His-tag). In most gel systems, PsbA migrates between 30 and 37 kDa
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