IL-6 Protein

Catalog No. ABIN988086

Product Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQanalysi s The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Pro-Pro. p to grams level.

Characteristics: The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to aof 5×107 IU/mg

Purity: > 98.0 % as determined by:(a) Analysis by RP-HPLC(b) Anion-exchange FPLC(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Endotoxin Level: Level Less than 0.1ng/µg (1 IEU/µg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Recombinant Human Interleukin-6 (IL-6) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of Interleukin-6 (IL-6) as a Reference Standard.

Storage: -20 °C

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: Interleukin-6 (IL-6) (IL6 Products)

Synonyms: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Background: Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-beta-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Human Interleukin-6 (IL-6) produced in E. coli is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da. The Recombinant Human Interleukin-6 (IL-6) is purified by proprietary chromatographic techniques. Synonym: rHuIL-6, rHuIL6, Interleukin-6, Interleukin. Formulation: The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.

Molecular Weight: 21,000 Da

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome