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GFAP antibody

GFAP Reactivity: Human, Rat, Pig WB, IHC (p), ICC, IP Host: Mouse Monoclonal GA-5 unconjugated
Catalog No. ABIN457420
  • Target See all GFAP Antibodies
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Reactivity
    • 292
    • 172
    • 164
    • 49
    • 49
    • 33
    • 30
    • 11
    • 9
    • 9
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Pig
    Host
    • 194
    • 138
    • 12
    • 3
    • 2
    • 1
    Mouse
    Clonality
    • 195
    • 155
    Monoclonal
    Conjugate
    • 161
    • 29
    • 25
    • 19
    • 13
    • 9
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This GFAP antibody is un-conjugated
    Application
    • 283
    • 129
    • 101
    • 83
    • 73
    • 62
    • 44
    • 29
    • 26
    • 26
    • 22
    • 20
    • 13
    • 8
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    The mouse monoclonal antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
    Cross-Reactivity (Details)
    Human, Porcine, Rat
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Porcine spinal cord
    Clone
    GA-5
    Isotype
    IgG1
    Top Product
    Discover our top product GFAP Primary Antibody
  • Application Notes
    Immunocytochemistry: Recommended dilution: 1-4 μg/mL.
    Western blotting: Recommended dilution: 1-2 μg/mL.
    Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/mL.
    Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein per 1 mL lysate.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Joardar, Sen, Das: "Docosahexaenoic acid facilitates cell maturation and beta-adrenergic transmission in astrocytes." in: Journal of lipid research, Vol. 47, Issue 3, pp. 571-81, (2006) (PubMed).

    Guillemin, Wang, Brew: "Quinolinic acid selectively induces apoptosis of human astrocytes: potential role in AIDS dementia complex." in: Journal of neuroinflammation, Vol. 2, pp. 16, (2005) (PubMed).

    Rungger-Brändle, Dosso, Leuenberger: "Glial reactivity, an early feature of diabetic retinopathy." in: Investigative ophthalmology & visual science, Vol. 41, Issue 7, pp. 1971-80, (2000) (PubMed).

    Perng, Cairns, van den IJssel, Prescott, Hutcheson, Quinlan: "Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin." in: Journal of cell science, Vol. 112 ( Pt 13), pp. 2099-112, (1999) (PubMed).

  • Target
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Alternative Name
    GFAP (GFAP Products)
    Synonyms
    GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
    Background
    Glial fibrillary acidic proteinprovided,GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.,GFAP, ALXDRD
    Gene ID
    2670
    UniProt
    P14136
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