p53 antibody (AA 364-393)

Catalog No. ABIN1882106

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: AA 364-393

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.

Clone: RB2571

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-p53 antibody (ABIN1882106)

Chen, Chen: "MDM2-ARF complex regulates p53 sumoylation." in: Oncogene, Vol. 22, Issue 34, pp. 5348-57, (2003) (PubMed).

Melchior, Hengst: "SUMO-1 and p53." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 245-9, (2002) (PubMed).

Muller, Berger, Lehembre, Seeler, Haupt, Dejean: "c-Jun and p53 activity is modulated by SUMO-1 modification." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13321-9, (2000) (PubMed).

Gostissa, Hengstermann, Fogal, Sandy, Schwarz, Scheffner, Del Sal: "Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1." in: The EMBO journal, Vol. 18, Issue 22, pp. 6462-71, (2000) (PubMed).

Rodriguez, Desterro, Lain, Midgley, Lane, Hay: "SUMO-1 modification activates the transcriptional response of p53." in: The EMBO journal, Vol. 18, Issue 22, pp. 6455-61, (2000) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53 Products)

Synonyms: BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody

Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Molecular Weight: 43653

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect