Neutrophils antibody

Catalog No. ABIN1169411

Product Details anti-Neutrophils Antibody

Target: Neutrophils

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Neutrophils antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunocytochemistry (ICC)

Specificity: Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).

Cross-Reactivity: Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Purified mouse BALB/c neutrophils.

Clone: Nimp-R14

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Negative control: AG-35B-0011 http://www.adipogen.com/ag-35b-0011/rat-igg2b-isotype-control.html

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Neutrophils antibody (ABIN1169411)

Yoshida, Taguchi, Kawana, Ogishima, Adachi, Kawata, Nakamura, Sato, Fujimoto, Inoue, Tomio, Mori, Nagamatsu, Arimoto, Koga, Hiraike, Oda, Kiyono, Osuga, Fujii: "Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity." in: International journal of oncology, Vol. 53, Issue 4, pp. 1580-1590, (2018) (PubMed).

Henderson, Hobbs, Mathies, Hogg: "Rapid recruitment of inflammatory monocytes is independent of neutrophil migration." in: Blood, Vol. 102, Issue 1, pp. 328-35, (2003) (PubMed).

de Vries, Köhl, Leclercq, Wolfs, van Bijnen, Heeringa, Buurman: "Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 7, pp. 3883-9, (2003) (PubMed).

Tacchini-Cottier, Zweifel, Belkaid, Mukankundiye, Vasei, Launois, Milon, Louis: "An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 5, pp. 2628-36, (2000) (PubMed).

Al-Qaoud, Pearlman, Hartung, Klukowski, Fleischer, Hoerauf: "A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5." in: International immunology, Vol. 12, Issue 6, pp. 899-908, (2000) (PubMed).

Lubberts, Joosten, van Den Bersselaar, Helsen, Bakker, van Meurs, Graham, Richards, van Den Berg: "Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4546-56, (1999) (PubMed).

Van Lent, Blom, Holthuysen, Jacobs, Van De Putte, Van Den Berg: "Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritis in mice." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 267-78, (1997) (PubMed).

Lopez, Strath, Sanderson: "Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies." in: British journal of haematology, Vol. 57, Issue 3, pp. 489-94, (1984) (PubMed).

Target Details for Neutrophils

Target: Neutrophils

Abstract: Neutrophils Products

Background: Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.

UniProt: P35461