IGF1R-beta antibody

Catalog No. ABIN1724670

Product Details

Target: IGF1R-beta

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Purification: purified

Immunogen: Purified recombinant fragment of IGF1R-Beta expressed in E. coli.

Clone: 3C8B1

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References

Scheidegger, Cenni, Picard, Delafontaine: "Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." in: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, (2001) (PubMed).

Adams, Epa, Garrett, Ward: "Structure and function of the type 1 insulin-like growth factor receptor." in: Cellular and molecular life sciences : CMLS, Vol. 57, Issue 7, pp. 1050-93, (2000) (PubMed).

Target Details

Target: IGF1R-beta

Background:

Description: IGF1R (insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Aliases: IGF1R, IGF1R-Beta

Molecular Weight: 96 kDa

Gene ID: 3480

HGNC: 3480