NPT antibody

Catalog No. ABIN969318

Product Details

Target: NPT

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: purified

Immunogen: Purified recombinant fragment of NPT expressed in E. coli.

Clone: 4B4D1

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References

Lukasik, Bradley, Scott, Dea, Koo, Hsu, Bartz, Farrah: "Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Target Details

Target: NPT

Background:

Description: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Aliases: N/A