CD31 antibody (FITC)

Catalog No. ABIN94071

Product Details anti-CD31 Antibody (FITC)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Leukocytes of a patient suffering from LGL-type leukaemia

Clone: MEM-05

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD31 antibody (FITC) (ABIN94071)

Prager, Staffler, Majdic, Säemann, Godár, Zlabinger, Stockinger: "Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 4, pp. 2364-71, (2001) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background: Platelet and endothelial cell adhesion molecule 1,CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.,PECAM-1, EndoCAM, GPIIA, PECA1

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization