Surfactant Protein C Cross Reactive with Mouse Sp-C antibody

Catalog No. ABIN2192205

Product Details

Target: Surfactant Protein C Cross Reactive with Mouse Sp-C

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Nogee, Dunbar, Wert, Askin, Hamvas, Whitsett: "A mutation in the surfactant protein C gene associated with familial interstitial lung disease." in: The New England journal of medicine, Vol. 344, Issue 8, pp. 573-9, (2001) (PubMed).

Ross, Ikegami, Steinhilber, Jobe: "Surfactant protein C in fetal and ventilated preterm rabbit lungs." in: The American journal of physiology, Vol. 277, Issue 6 Pt 1, pp. L1104-8, (2000) (PubMed).

Nogee, Wert, Proffit, Hull, Whitsett: "Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 3 Pt 1, pp. 973-81, (2000) (PubMed).

Target Details

Target: Surfactant Protein C Cross Reactive with Mouse Sp-C

Background: The polyclonal antibody recognizes the human surfactant protein C (SP-C). There are four surfactant- specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-C is a 34-35 amino acid peptide, of 4 kD that is proteolytically processed from a 21 kD precursor protein. SP-C is initiated early in the embryogenic period of lung formation, where SP-C transcripts are detected uniformly in epithelial cells lining the primitive airways. During lung development SP-C expression is decreased in cells of the proximal conducting portion of the lung. Ultimately SP-C is expressed selectively in type II epithelial cells in the alveolus of the lung. SP-C is secreted into the airspace where it enhances the spreading and