VCAM1 antibody

Catalog No. ABIN2191851

Product Details anti-VCAM1 Antibody

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Sterility: 0.2 μm filtered

Clone: 6C7-1

Application Details

Application Notes: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-VCAM1 antibody (ABIN2191851)

Kuligowski, Kwan, Lo, Wong, James, Bourges, Ooi, Abeynaike, Hall, Kitching, Hickey: "Antimyeloperoxidase antibodies rapidly induce alpha-4-integrin-dependent glomerular neutrophil adhesion." in: Blood, Vol. 113, Issue 25, pp. 6485-94, (2009) (PubMed).

Kneilling, Mailhammer, Hültner, Schönberger, Fuchs, Schaller, Bukala, Massberg, Sander, Braumüller, Eichner, Maier, Hallmann, Pichler, Haubner, Gawaz, Pfeffer, Biedermann, Röcken: "Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation." in: Blood, Vol. 114, Issue 8, pp. 1696-706, (2009) (PubMed).

Vajkoczy, Laschinger, Engelhardt: "Alpha4-integrin-VCAM-1 binding mediates G protein-independent capture of encephalitogenic T cell blasts to CNS white matter microvessels." in: The Journal of clinical investigation, Vol. 108, Issue 4, pp. 557-65, (2001) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: Vcam-1 (VCAM1 Products)

Synonyms: CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody

Background: The monoclonal antibody 6C7.1 recognizes mouse vascular cell adhesion molecule (VCAM-1) (~ 81 kDa), a member of a subclass of the immunoglobulin superfamily (IgSF). IgSF members are ligands for integrins. Cell adhesion molecules (CAMs) have important roles in the immune response, immune surveillance and cell-cell recognition, especially in leukocyte-endothelial cell adhesion. CAMs on the surface of leukocytes and endothelial cells are actively involved in the recruitment of specific leukocyte subsets into different tissues. VCAM-1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Cell adhesion molecules, like VCAM-1, are upregulated on cerebral vessels during inflammatory conditions of the central nervous system such as experimental autoimmune encephalomyelitis (EAE), a model system for multiple sclerosis. Administration of monoclonal antibody 6C7.1 has been shown to inhibit or diminish clinical or pathological signs of EAE. VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells. Expression of VCAM-1 on vascular endothelial cells is induced by TNF-alpha, IL-1, IFN-gamma or endotoxin. VCAM-1 is a ligand for the integrins alpha4beta1 (VLA-4) and alpha4beta7 (LPAM-1). These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. VCAM-1/VLA-4 interaction may play a pathophysiological role in immune responses and as well as in leukocyte emigration to sites of inflammation. CD106, INCAM-100, Vascular cell adhesion protein 1 Aliases Mouse endothelial cells Immunogen rat IgG1

Pathways: Carbohydrate Homeostasis