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HCV 1b NS5B antibody

HCV NS5b Reactivity: Hepatitis C Virus (HCV) IP, ICC, Func Host: Mouse Monoclonal 5B-12B7 unconjugated
Catalog No. ABIN1169140
  • Target See all HCV 1b NS5B (HCV NS5b) products
    HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
    Reactivity
    • 36
    • 16
    Hepatitis C Virus (HCV)
    Host
    • 20
    • 16
    • 1
    Mouse
    Clonality
    • 20
    • 17
    Monoclonal
    Conjugate
    • 23
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HCV 1b NS5B antibody is un-conjugated
    Application
    • 33
    • 22
    • 16
    • 13
    • 13
    • 9
    • 3
    • 3
    • 1
    • 1
    Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)
    Specificity
    Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392).
    Cross-Reactivity
    Hepatitis C Virus (HCV)
    Cross-Reactivity (Details)
    Does not react with genotype 2.
    Purification
    Purified from concentrated hybridoma tissue culture supernatant.
    Purity
    >95 % (SDS-PAGE)
    Immunogen
    Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
    Clone
    5B-12B7
    Isotype
    IgG2a
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    In PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 year after receipt when stored at -20°C.
    Expiry Date
    12 months
  • Dimitrova, Imbert, Kieny, Schuster: "Protein-protein interactions between hepatitis C virus nonstructural proteins." in: Journal of virology, Vol. 77, Issue 9, pp. 5401-14, (2003) (PubMed).

    Kapadia, Brideau-Andersen, Chisari: "Interference of hepatitis C virus RNA replication by short interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 2014-8, (2003) (PubMed).

    Moradpour, Bieck, Hügle, Wels, Wu, Hong, Blum, Bartenschlager: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).

    Schmidt-Mende, Bieck, Hugle, Penin, Rice, Blum, Moradpour: "Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 44052-63, (2001) (PubMed).

  • Target
    HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
    Abstract
    HCV NS5b Products
    Target Type
    Viral Protein
    Background
    The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
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