LYN antibody (AA 8-238)

Catalog No. ABIN120725

Product Details anti-LYN Antibody

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Binding Specificity: AA 8-238

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This LYN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Specificity: The antibody reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.

Purification: Protein A affinity chromatography

Immunogen: Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238)

Clone: LYN-01

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Immunocytochemistry. Western Blotting: Recommended dilution: 1-2 μg/mL, 60 min. Positive control: RBL rat basophilic leukemia cell line JURKAT human peripheral blood leukemia T-cell line A-431 human epidermoid carcinoma cell line U-937 human histiocytic lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-LYN antibody (ABIN120725)

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Kovárová, Tolar, Arudchandran, Dráberová, Rivera, Dráber: "Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8318-28, (2001) (PubMed).

Amoui, Dráberová, Tolar, Dráber: "Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors." in: European journal of immunology, Vol. 27, Issue 1, pp. 321-8, (1997) (PubMed).

Target Details for LYN

Target: LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

Alternative Name: LYN (LYN Products)

Synonyms: JTK8 antibody, p53Lyn antibody, p56Lyn antibody, lyn antibody, LYN antibody, AA407514 antibody, Hck-2 antibody, jtk8 antibody, lyn-A antibody, CH73-38P6.3 antibody, zgc:92124 antibody, LYN proto-oncogene, Src family tyrosine kinase antibody, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog antibody, tyrosine-protein kinase Lyn antibody, LYN proto-oncogene, Src family tyrosine kinase L homeolog antibody, LYN antibody, lyn antibody, Lyn antibody, LOC100593961 antibody, lyn.L antibody

Background: Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3. Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).Synonyms: Tyrosine-protein kinase Lyn

Gene ID: 4067

UniProt: P07948

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling