E2F1 antibody (pSer364)

Catalog No. ABIN3201010

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: pSer364

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Product: Mouse monoclonal antibody (clone #2) specific to the human E2F1 protein phosphorylated at Ser364. Produced in serum-free medium and purified under mild conditions

Purification: Purified

Immunogen: A synthetic peptide corresponding to a sequence of human E2F1 protein including and surrounding pSer364

Clone: #2

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. ELISA
Not tested for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (-), 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-E2F1 antibody (ABIN3201010)

Trimarchi, Lees: "Sibling rivalry in the E2F family." in: Nature reviews. Molecular cell biology, Vol. 3, Issue 1, pp. 11-20, (2002) (PubMed).

Irwin, Marin, Phillips, Seelan, Smith, Liu, Flores, Tsai, Jacks, Vousden, Kaelin: "Role for the p53 homologue p73 in E2F-1-induced apoptosis." in: Nature, Vol. 407, Issue 6804, pp. 645-8, (2000) (PubMed).

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1 (E2F1 Products)

Synonyms: CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody

Background: E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor. E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor. It induces apoptosis by activating transcription of the p53 homolog, p73. E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy