HIV-1 Nef (full length) antibody

Catalog No. ABIN2452025

Product Details

Target: HIV-1 Nef

Binding Specificity: full length

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Characteristics: Whole Antiserum

Purification: Guinea Pig serum

Immunogen: Purified full-size recombinant Nef of HIV-1 subtype B expressed in E. coli

Application Details

Application Notes: 1. Western blot: 1/1,000~1/3,000
2. Dot blot: 1/3000
3. Immunoprecipitation (assay dependent)
4. ELISA (assay dependent)
Other applications have not been tested

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Upon receipt aliquot and store at -20 C

References

Arora, Fredericksen, Garcia: "Nef: agent of cell subversion." in: Microbes and infection / Institut Pasteur, Vol. 4, Issue 2, pp. 189-99, (2002) (PubMed).

Fackler, Baur: "Live and let die: Nef functions beyond HIV replication." in: Immunity, Vol. 16, Issue 4, pp. 493-7, (2002) (PubMed).

Target Details

Target: HIV-1 Nef

Target Type: Viral Protein

Background: HIV-1 Nef (27 kDa) is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.