TNFSF13 Protein (AA 16-108, Extracellular Domain) (DYKDDDDK Tag)

Catalog No. ABIN1344412

Product Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 16-108, Extracellular Domain

Origin: Human, Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF13 protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Brand: MegaAPRIL™

Specificity: Binds to human and mouse BCMA and TACI. Binds to proteoglycans.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Stimulates B cell proliferation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

References

Rodríguez-Bayona, Ramos-Amaya, López-Blanco, Campos-Caro, Brieva: "STAT-3 activation by differential cytokines is critical for human in vivo-generated plasma cell survival and Ig secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4996-5004, (2013) (PubMed).

Sakurai, Hase, Kanno, Kojima, Okumura, Kobata: "TACI regulates IgA production by APRIL in collaboration with HSPG." in: Blood, Vol. 109, Issue 7, pp. 2961-7, (2007) (PubMed).

Endo, Nishio, Enzler, Cottam, Fukuda, James, Karin, Kipps: "BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway." in: Blood, Vol. 109, Issue 2, pp. 703-10, (2007) (PubMed).

Mongini, Inman, Han, Fattah, Abramson, Attur: "APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 11, pp. 6736-51, (2006) (PubMed).

Craxton, Draves, Gruppi, Clark: "BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway." in: The Journal of experimental medicine, Vol. 202, Issue 10, pp. 1363-74, (2005) (PubMed).

Bonci, Hahne, Felli, Peschle, De Maria: "Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis." in: Blood, Vol. 104, Issue 10, pp. 3169-72, (2004) (PubMed).

He, Chadburn, Jou, Schattner, Knowles, Cerutti: "Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 5, pp. 3268-79, (2004) (PubMed).

Litinskiy, Nardelli, Hilbert, He, Schaffer, Casali, Cerutti: "DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL." in: Nature immunology, Vol. 3, Issue 9, pp. 822-9, (2002) (PubMed).

Stein, López-Fraga, Elustondo, Carvalho-Pinto, Rodríguez, Gómez-Caro, De Jong, Martínez-A, Medema, Hahne: "APRIL modulates B and T cell immunity." in: The Journal of clinical investigation, Vol. 109, Issue 12, pp. 1587-98, (2002) (PubMed).

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL (TNFSF13 Products)

Synonyms: APRIL Protein, CD256 Protein, TALL-2 Protein, TALL2 Protein, TRDL-1 Protein, ZTNF2 Protein, 2310026N09Rik Protein, April Protein, Tall2 Protein, Trdl1 Protein, SLAN Protein, ANP32B Protein, april Protein, tnf13 Protein, TNLG7B Protein, TNF superfamily member 13 Protein, tumor necrosis factor (ligand) superfamily, member 13 Protein, acidic leucine-rich nuclear phosphoprotein 32 family member B Protein, TNFSF13 Protein, Tnfsf13 Protein, LOC494442 Protein, tnfsf13 Protein

Background: APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the NH2 terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Molecular Weight: ~34kDa (SDS-PAGE)

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response