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CD20 antibody (PerCP)

MS4A1 Reactivity: Human FACS, IF Host: Mouse Monoclonal LT20 PerCP
Catalog No. ABIN1112087
  • Target See all CD20 (MS4A1) Antibodies
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Reactivity
    • 259
    • 57
    • 23
    • 18
    • 17
    • 16
    • 11
    • 7
    • 6
    • 5
    • 2
    • 2
    Human
    Host
    • 190
    • 96
    • 2
    • 2
    Mouse
    Clonality
    • 208
    • 80
    • 1
    Monoclonal
    Conjugate
    • 108
    • 21
    • 18
    • 16
    • 13
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD20 antibody is conjugated to PerCP
    Application
    • 218
    • 93
    • 76
    • 47
    • 46
    • 38
    • 25
    • 20
    • 20
    • 16
    • 14
    • 13
    • 11
    • 11
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Characteristics
    Mouse Monoclonal Anti-Human CD20 PE is recommended for use in flow cytometry for identification of B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow..
    Clone
    LT20
    Isotype
    IgG1
    Top Product
    Discover our top product MS4A1 Primary Antibody
  • Application Notes
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    Comment

    Peridin-cholophyll-protein complex (Prozyme).

    Sample Collection
    1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 20 µl of CD20 PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing Antibody Stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Storage
    4 °C
  • Cragg, Morgan, Chan, Morgan, Filatov, Johnson, French, Glennie: "Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts." in: Blood, Vol. 101, Issue 3, pp. 1045-52, (2003) (PubMed).

    Orfao, Ciudad, Gonzalez, Lopez, del Mar Abad, Paz Bouza, Cruz, Gomez Alonso, San Miguel: "Flow cytometry in the diagnosis of cancer." in: Scandinavian journal of clinical and laboratory investigation. Supplementum, Vol. 221, pp. 145-52, (1995) (PubMed).

  • Target
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Alternative Name
    CD20 (MS4A1 Products)
    Synonyms
    B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
    Background
    This antibody recognizes the 33-37 kDa molecular weight membrane-embedded non- glycosylated phosphoprotein made up of two polypeptide chains. CD20 is present on all normal B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow. The antigen is also present on most B cell lines and on non-T ALL and CLL cells.
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