MLLT10 antibody (AA 294-323)

Catalog No. ABIN1882102

Product Details anti-MLLT10 Antibody

Target: MLLT10 (Protein AF-10 (MLLT10))

Binding Specificity: AA 294-323

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLLT10 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MLLT10 (AF10) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human MLLT10 (AF10).

Clone: RB8383

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MLLT10 antibody (ABIN1882102)

Benedikt, Baltruschat, Scholz, Bursen, Arrey, Meyer, Varagnolo, Müller, Karas, Dingermann, Marschalek: "The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures." in: Leukemia, Vol. 25, Issue 1, pp. 135-44, (2011) (PubMed).

Cai, Gao, Sheng, Miao, Cui, Wang, Zong, Koide: "Characterization and potential function of a novel testis-specific nucleoporin BS-63." in: Molecular reproduction and development, Vol. 61, Issue 1, pp. 126-34, (2002) (PubMed).

Perrin, Bloyer, Ferraz, Agrawal, Sinha, Dura: "The leucine zipper motif of the Drosophila AF10 homologue can inhibit PRE-mediated repression: implications for leukemogenic activity of human MLL-AF10 fusions." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 119-30, (2002) (PubMed).

Roll, Zattara-Cannoni, Bustos-Bernard, Curtillet, Michel, Vagner-Capodano: "Molecular and fluorescence in situ hybridization analysis of a 10;11 rearrangement in a case of infant acute monocytic leukemia." in: Cancer genetics and cytogenetics, Vol. 135, Issue 2, pp. 187-91, (2002) (PubMed).

Nakamura, Mori, Tada, Krajewski, Rozovskaia, Wassell, Dubois, Mazo, Croce, Canaani: "ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation." in: Molecular cell, Vol. 10, Issue 5, pp. 1119-28, (2002) (PubMed).

Debernardi, Bassini, Jones, Chaplin, Linder, de Bruijn, Meese, Young: "The MLL fusion partner AF10 binds GAS41, a protein that interacts with the human SWI/SNF complex." in: Blood, Vol. 99, Issue 1, pp. 275-81, (2001) (PubMed).

Target Details for MLLT10

Target: MLLT10 (Protein AF-10 (MLLT10))

Alternative Name: MLLT10 (AF10) (MLLT10 Products)

Synonyms: AF10 antibody, Af10 antibody, B130021D15Rik antibody, D630001B22Rik antibody, mKIAA4140 antibody, MLLT10, histone lysine methyltransferase DOT1L cofactor antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10 antibody, MLLT10 antibody, Mllt10 antibody

Background: Translocations affecting chromosome 11q23 involve many partner chromosome regions and occur in various leukemias. The 11q23 gene involved in the translocations is MLL. MLLT10 is the partner gene to MLL1 involved in t(10,11)(p12,q23) translocations. In an analysis of two leukemia patients, the in t(10,11)(p12,q23) translocation fuses MLL1, a SET domain containg histone methyltransferase, to the MLLT10 gene. The MLLT10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain. The MLLT10 gene is one of the few MLL partner genes to be independently rearranged with a third gene in leukemia, the CALM gene in the t(10,11)(p12,q14) translocation. Chimeric fusion proteins MLL/AF10 and CALM/AF10 consistently retain the leucine zipper motif of MLLT10. The leucine zipper interacts with GAS41, a protein previously identified as the product of an amplified gene in a glioblastoma. GAS41 interacts with integrase interactor-1 (INI1), a component of the SWI/SNF complex, which acts to remodel chromatin and to modulate transcription. Retention of the leucine zipper in the MLL and CALM fusions suggested that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate-dependent chromatin remodeling complexes.

Molecular Weight: 113320

NCBI Accession: NP_001182555, NP_001182556, NP_001182557, NP_001182559, NP_004632

UniProt: P55197