LIMA1 antibody (Middle Region)

Catalog No. ABIN2775866

Product Details anti-LIMA1 Antibody

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 92%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against LIMA1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human LIMA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 759 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-LIMA1 antibody (ABIN2775866)

Maul, Song, Amann, Gerbin, Pollard, Chang: "EPLIN regulates actin dynamics by cross-linking and stabilizing filaments." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 399-407, (2003) (PubMed).

Target Details for LIMA1

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Alternative Name: LIMA1 (LIMA1 Products)

Synonyms: eplin antibody, LIMA1 antibody, EPLIN antibody, MGC146397 antibody, SREBP3 antibody, 1110021C24Rik antibody, 3526402A12Rik antibody, D15Ertd366e antibody, Eplin antibody, LIM domain and actin binding 1 antibody, Lima1 antibody, LIMA1 antibody, lima1 antibody

Background: This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.
Alias Symbols: EPLIN, SREBP3
Protein Interaction Partner: TP53, UBC, RPA3, RPA2, RPA1, SUZ12, RNF2, EZH2, BMI1, LRCH3, YWHAQ, YWHAB, PAN2, YWHAE, FMNL1, PAXIP1, ATP6V1B1, APP, UBXN6, LRRK2, ARRB2, ARRB1, SVIL, YWHAG,
Protein Size: 759

Molecular Weight: 85 kDa

Gene ID: 51474

NCBI Accession: NM_016357, NP_057441

UniProt: Q9UHB6