CD34 antibody

Catalog No. ABIN2191756

Product Details anti-CD34 Antibody

Target: CD34

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Sterility: 0.2 μm filtered

Clone: MEC14-7

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive H5V cells control Negative Muscle cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD34 antibody (ABIN2191756)

Sho, Sho, Nanjo, Kawamura, Masuda, Dalman: "Hemodynamic regulation of CD34+ cell localization and differentiation in experimental aneurysms." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 10, pp. 1916-21, (2004) (PubMed).

Solberg, Rinkenberger, Danø, Werb, Lund: "A functional overlap of plasminogen and MMPs regulates vascularization during placental development." in: Development (Cambridge, England), Vol. 130, Issue 18, pp. 4439-50, (2003) (PubMed).

Almholt, Nielsen, Frandsen, Brünner, Danø, Johnsen: "Metastasis of transgenic breast cancer in plasminogen activator inhibitor-1 gene-deficient mice." in: Oncogene, Vol. 22, Issue 28, pp. 4389-97, (2003) (PubMed).

Garlanda, Berthier, Garin, Stoppacciaro, Ruco, Vittet, Gulino, Matteucci, Mantovani, Vecchi, Dejana: "Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors." in: European journal of cell biology, Vol. 73, Issue 4, pp. 368-77, (1997) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: The monoclonal antibody MEC14.7 recognizes mouse CD34, a single-pass type I membrane glycophosphoprotein present on small vessel endothelial cells and hematopoietic progenitor cells. The apparent molecular mass of CD34 is heterogeneous, depending on the glycosylation state in different cell types. In cultured endothelioma cell lysate, CD34 has a molecular weight of ~100 kDa, whereas in lung lysates it is ~80 kDa. 2 Isoforms of CD34 exist, both are expressed on the cell surface.CD34 is an adhesion molecule performing a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 acts as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 presents carbohydrate ligands to selectins.CD34 is widely used as a marker to select early hematopoietic stem and progenitor cells in experimental and clinical hematopoiesis. The monoclonal antibody MEC14.7 recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. The monoclonal antibody MEC14.7 can be used for identification and characterization of capillary endothelial cells. Furthermore, the antibody is useful for isolation and characterization of hematopoietic progenitor cells, particularly of myelomonocytic colony forming cells. Monoclonal antibody MEC14.7 is also useful for immunopurification and cell separation Aliases Hematopoietic progenitor cell antigen CD34 Immunogen Murine transformed EC t-end.1