Shugoshin antibody

Catalog No. ABIN2452123

Product Details anti-Shugoshin Antibody

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Schizosaccharomyces pombe

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Purification: Affinity purified

Sterility: Sterile filtered

Application Details

Application Notes: 1) Western blotting: 1,000 fold dilution
2) Immunofluoresece staining: 100 fold dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1 mg/mL BSA, PBS ( pH 7.2), 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-Shugoshin antibody (ABIN2452123)

Yamagishi, Sakuno, Shimura, Watanabe: "Heterochromatin links to centromeric protection by recruiting shugoshin." in: Nature, Vol. 455, Issue 7210, pp. 251-5, (2008) (PubMed).

Watanabe, Kitajima: "Shugoshin protects cohesin complexes at centromeres." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 360, Issue 1455, pp. 515-21, discussion 521, (2005) (PubMed).

Kitajima, Kawashima, Watanabe: "The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis." in: Nature, Vol. 427, Issue 6974, pp. 510-7, (2004) (PubMed).

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Abstract: SGOL1 Products

Background: Shugoshin 1 (Sgo1), a meiosis-specific protein, protects centromeric Rec8 from degradation during first meiotic division (meiosis I) in fission yeast Schizosaccharomyces pombe. This protein appears at late prophase of meiosis I and is degraded at anaphase I. Heterochromatin protein Swi6 associates directly with Sgo1. A point mutation of Sgo1 (V242E), which abolishes the interaction with Swi6, impairs the centromeric localization and function of Sgo1. The forced centromeric localization of Sgo1 restores proper meiotic chromosome segregation in swi6 cells. Using this antibody in Western blotting, the band of 37 kD was observed in wild type S. pombe MP111 extract.

UniProt: Q9P7A0

Pathways: Maintenance of Protein Location