TNFRSF8 antibody

Catalog No. ABIN2749180

Product Details anti-TNFRSF8 Antibody

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody Ber-H8 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells, it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Clone: Ber-H8

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNFRSF8 antibody (ABIN2749180)

Aalberse, Kapitein, de Roock, Klein, de Jager, van der Zee, Hoekstra, van Wijk, Prakken: "Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60)." in: PLoS ONE, Vol. 6, Issue 9, pp. e24119, (2011) (PubMed).

Matsumoto, Terakawa, Miura, Fukuda, Nakajima, Saito: "Extremely rapid and intense induction of apoptosis in human eosinophils by anti-CD30 antibody treatment in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 4, pp. 2186-93, (2004) (PubMed).

Berro, Perry, Agrawal: "Increased expression and activation of CD30 induce apoptosis in human blood eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 3, pp. 2174-83, (2004) (PubMed).

Franke, Jung, Ellis: "Characterization of the CD30L binding domain on the human CD30 molecule using anti-CD30 antibodies." in: Hybridoma, Vol. 19, Issue 1, pp. 43-8, (2000) (PubMed).

Falini, Pileri, Pizzolo, Dürkop, Flenghi, Stirpe, Martelli, Stein: "CD30 (Ki-1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy." in: Blood, Vol. 85, Issue 1, pp. 1-14, (1995) (PubMed).

Target Details for TNFRSF8

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Alternative Name: CD30 (TNFRSF8 Products)

Synonyms: CD30 antibody, D1S166E antibody, Ki-1 antibody, Cd30 antibody, Ki antibody, TNFRSF8 antibody, TNF receptor superfamily member 8 antibody, tumor necrosis factor receptor superfamily, member 8 antibody, TNFRSF8 antibody, Tnfrsf8 antibody

Background: TNF receptor superfamily member 8,CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.,Ki-1, TNFRSF8, CD30L receptor, D1S166E

Gene ID: 943

UniProt: P28908