Tuberin antibody (pSer1387)

Catalog No. ABIN1881949

Product Details anti-Tuberin Antibody

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Binding Specificity: pSer1387

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: Dot Blot (DB), Immunofluorescence (IF)

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This TSC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1387 of human TSC2.

Clone: RB11388

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Tuberin antibody (ABIN1881949)

Huang, Ratovitski: "Phospho-?Np63?/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells." in: Aging, Vol. 2, Issue 12, pp. 959-68, (2011) (PubMed).

Li, Inoki, Guan: "Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 7965-75, (2004) (PubMed).

Karbowniczek, Cash, Cheung, Robertson, Astrinidis, Henske: "Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 29930-7, (2004) (PubMed).

Corradetti, Inoki, Bardeesy, DePinho, Guan: "Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome." in: Genes & development, Vol. 18, Issue 13, pp. 1533-8, (2004) (PubMed).

Birchenall-Roberts, Fu, Bang, Dambach, Resau, Sadowski, Bertolette, Lee, Kim, Ruscetti: "Tuberous sclerosis complex 2 gene product interacts with human SMAD proteins. A molecular link of two tumor suppressor pathways." in: The Journal of biological chemistry, Vol. 279, Issue 24, pp. 25605-13, (2004) (PubMed).

Lewis, Thomas, Murphy, Sampson: "Genotype and psychological phenotype in tuberous sclerosis." in: Journal of medical genetics, Vol. 41, Issue 3, pp. 203-7, (2004) (PubMed).

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: TSC2 (TSC2 Products)

Background: Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.

Molecular Weight: 200608

NCBI Accession: NP_000539, NP_001070651, NP_001107854

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus