GLO1 antibody (full length)

Catalog No. ABIN2451990

Product Details anti-GLO1 Antibody

Target: GLO1 (Glyoxalase I (GLO1))

Binding Specificity: full length

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunochromatography (IC)

Specificity: Specific to human, simian, and mouse glyoxalase I.

Cross-Reactivity (Details): Not tested in other species.

Characteristics: The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused mouse glyoxalase I (full length)

Clone: 6F10

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: ~ 1,000
2. Immunocytochemistry
3. ELISA
Other applications are not tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-GLO1 antibody (ABIN2451990)

Hovatta, Tennant, Helton, Marr, Singer, Redwine, Ellison, Schadt, Verma, Lockhart, Barlow: "Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice." in: Nature, Vol. 438, Issue 7068, pp. 662-6, (2005) (PubMed).

Chen, Wollmer, Hoerndli, Münch, Kuhla, Rogaev, Tsolaki, Papassotiropoulos, Götz: "Role for glyoxalase I in Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 20, pp. 7687-92, (2004) (PubMed).

Junaid, Kowal, Barua, Pullarkat, Sklower Brooks, Pullarkat: "Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor." in: American journal of medical genetics. Part A, Vol. 131, Issue 1, pp. 11-7, (2004) (PubMed).

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: Glyoxalase 1 (GLO1) (GLO1 Products)

Synonyms: GLOD1 antibody, GLYI antibody, 0610009E22Rik antibody, 1110008E19Rik antibody, 2510049H23Rik antibody, AW550643 antibody, GLY1 antibody, Glo-1 antibody, Glo-1r antibody, Glo-1s antibody, Glo1-r antibody, Glo1-s antibody, Qglo antibody, cb554 antibody, zgc:66035 antibody, wu:fb82g09 antibody, glod1 antibody, glyi antibody, glo1 antibody, GLO1 antibody, ATGLX1 antibody, F12F1.32 antibody, F12F1_32 antibody, GLYOXALASE I antibody, glyoxalase I homolog antibody, trypanothione-dependent glyoxalase I antibody, glyoxalase I antibody, glyoxalase 1 antibody, glyoxalase 1 L homeolog antibody, glyoxalase 1 S homeolog antibody, lactoylglutathione lyase antibody, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein antibody, GLO1 antibody, Glo1 antibody, glo1 antibody, glo1.L antibody, glo1.S antibody, GST3 antibody, GLX1 antibody, STY1687 antibody, Bcen_2094 antibody, gloA antibody

Background: Glyoxalase I (GLO1) is an enzyme that plays a role in the detoxification of methylglyoxal (MG), a side-product of glycolysis, via condensation with glutathione to produce S-lactoyl-glutathione. GLO1 is a zinc metalloenzyme whose crystal structure has been solved. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric and its sequence is well conserved. GLO1 is found over-expressed in some tumors. GLO1 has also been suggested to be involved in anxiety diseases, autism, and Alzheimer's disease.

UniProt: Q9CPU0