Fascin antibody (AA 93-142)

Catalog No. ABIN6746004

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Binding Specificity: AA 93-142

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio), Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse FSCN1 / Fascin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig (100%) Bovine (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa93-142 of mouse Fscn1 (Q61553, NP_032010). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Horse, Pig, Guinea pig (100%), Panda, Bovine, Opossum (92%), Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Fascin antibody (ABIN6746004)

Dalle, Imamura, Rose, Worrall, Ugi, Hupfeld, Olefsky: "Insulin induces heterologous desensitization of G-protein-coupled receptor and insulin-like growth factor I signaling by downregulating beta-arrestin-1." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6272-85, (2002) (PubMed).

Attramadal, Arriza, Aoki, Dawson, Codina, Kwatra, Snyder, Caron, Lefkowitz: "Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family." in: The Journal of biological chemistry, Vol. 267, Issue 25, pp. 17882-90, (1992) (PubMed).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1 / Fascin (FSCN1 Products)

Synonyms: CG15331 antibody, CG1536 antibody, CG32858 antibody, Dmel\\CG32858 antibody, SN antibody, Sn antibody, fs(1)A1057 antibody, fs(1)K1421 antibody, fs(1)K418 antibody, fs(1)K473 antibody, fs(1)K743 antibody, fs(1)M45 antibody, p55 antibody, snl antibody, MGC75811 antibody, fscn1 antibody, sb:cb589 antibody, zgc:153632 antibody, AI663989 antibody, Fan1 antibody, fascin-1 antibody, Fascin antibody, fscn antibody, FAN1 antibody, HSN antibody, SNL antibody, fascin antibody, singed antibody, fascin actin-bundling protein 1 antibody, fascin actin-bundling protein 1a antibody, fascin actin-bundling protein 1 L homeolog antibody, sn antibody, fscn1 antibody, fscn1a antibody, Fscn1 antibody, fscn1.L antibody, FSCN1 antibody

Background: Name/Gene ID: FSCN1

Synonyms: FSCN1, 55 kDa actin-bundling protein, Fascin, HSN, Singed-like protein, SNL, p55

Gene ID: 6624

NCBI Accession: NP_032010

UniProt: Q16658