XPA antibody (full length)

Catalog No. ABIN3200998

Product Details anti-XPA Antibody

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Binding Specificity: full length

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Expected to react also with mouse XPA from the sequence homology.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant full-length human XPA protein

Clone: 5F12

Isotype: IgG2b

Application Details

Application Notes: 1. Western blotting: 0. 1~1 g/mL
2. ELISA
3. Inhibition of in vitro excision repair reaction
4. Inhibition of XPA interaction with ERCC1 and TFIIH
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-XPA antibody (ABIN3200998)

Saijo, Matsuda, Kuraoka, Tanaka: "Inhibition of nucleotide excision repair by anti-XPA monoclonal antibodies which interfere with binding to RPA, ERCC1, and TFIIH." in: Biochemical and biophysical research communications, Vol. 321, Issue 4, pp. 815-22, (2004) (PubMed).

Tanaka, Miura, Satokata, Miyamoto, Yoshida, Satoh, Kondo, Yasui, Okayama, Okada: "Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain." in: Nature, Vol. 348, Issue 6296, pp. 73-6, (1990) (PubMed).

Target Details for XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Alternative Name: XPA (XPA Products)

Synonyms: CG6358 antibody, DhR14 antibody, DhXPA antibody, DmXPA antibody, Dmel\\CG6358 antibody, Dxpa antibody, EG:EG0007.8 antibody, XPAC antibody, XPA_DROME antibody, Xpa antibody, dmXPA antibody, dxpa antibody, XP1 antibody, AI573865 antibody, Xpac antibody, xpac antibody, xxpa antibody, Xeroderma pigmentosum group A-like antibody, XPA, DNA damage recognition and repair factor antibody, xeroderma pigmentosum, complementation group A antibody, xeroderma pigmentosum, complementation group A L homeolog antibody, Xpac antibody, XPA antibody, Xpa antibody, xpa.L antibody

Background: XP (Xeroderma pigmentosum) is an autosomal recessive human disease characterized by hypersensitivity to sunlight and a high incidence of skin cancer on sun-exposed skin. Cells from XP patients are hypersensitive to killing by UV irradiation because of a defect in nucleotide excision repair (NER). XP is classified into seven complementation groups (A~G) and a variant form. XPA shows the most severe symptoms. Products encoded by the XP genes function in repairing UV-induced cyclobutane pyrimidine dimmer and (6-4) photoproducts as well as chemically induced variety of DNA lesions. XPA protein consists of 273 amino acids and forms a complex with many proteins such as RPA, ERCC1, TFIIH,XAB1, and XAB2, which plays a role in early step of NER. The hybridoma 5F12 was constructed by Prof. K. Tanaka's group who first cloned the XPA gene.

UniProt: P23025

Pathways: DNA Damage Repair