Piccolo antibody (AA 4439-4776)

Catalog No. ABIN1742311

Product Details anti-Piccolo Antibody

Target: Piccolo (PIC)

Binding Specificity: AA 4439-4776

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Piccolo antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for piccolo.

Purification: antiserum

Immunogen: Recombinant rat piccolo (aa 4439-4776).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (ECL detection)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500

Comment:

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. WB: This antibody detects an additional band of ~65 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Piccolo antibody (ABIN1742311)

Ribic, Zhang, Schlumbohm, Mätz-Rensing, Uchanska-Ziegler, Flügge, Zhang, Walter, Fuchs: "Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys." in: Cellular and molecular neurobiology, Vol. 30, Issue 6, pp. 827-39, (2010) (PubMed).

Matz, Gilyan, Kolar, McCarvill, Krueger: "Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 19, pp. 8836-41, (2010) (PubMed).

Szodorai, Kuan, Hunzelmann, Engel, Sakane, Sasaki, Takai, Kirsch, Müller, Beyreuther, Brady, Morfini, Kins: "APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 46, pp. 14534-44, (2009) (PubMed).

Tokoro, Higa, Deguchi-Tawarada, Inoue, Kitajima, Ohtsuka: "Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions." in: Neuroreport, Vol. 18, Issue 4, pp. 313-6, (2007) (PubMed).

Wahlin, Moreira, Huang, Yu, Adler: "Molecular dynamics of photoreceptor synapse formation in the developing chick retina." in: The Journal of comparative neurology, Vol. 506, Issue 5, pp. 822-37, (2007) (PubMed).

Dresbach, Torres, Wittenmayer, Altrock, Zamorano, Zuschratter, Nawrotzki, Ziv, Garner, Gundelfinger: "Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment." in: The Journal of biological chemistry, Vol. 281, Issue 9, pp. 6038-47, (2006) (PubMed).

Juranek, Mukherjee, Rickmann, Martens, Calka, Südhof, Jahn: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." in: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, (2006) (PubMed).

Haeberle, Fujiwara, Chuang, Medina, Panditrao, Bechstedt, Howard, Lumpkin: "Molecular profiling reveals synaptic release machinery in Merkel cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 40, pp. 14503-8, (2004) (PubMed).

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo (PIC Products)

Background: Synonyms: Aczonin