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HLA-ABC antibody

HLA-ABC Reactivity: Human, Cow, Cat, Monkey, Primate FACS, IP, IHC (fro), IF, EIA Host: Mouse Monoclonal W6-32 unconjugated
Catalog No. ABIN118900
  • Target See all HLA-ABC Antibodies
    HLA-ABC
    Reactivity
    • 69
    • 22
    • 14
    • 12
    • 12
    • 11
    • 10
    • 10
    • 8
    • 7
    • 6
    • 6
    • 1
    • 1
    • 1
    Human, Cow, Cat, Monkey, Primate
    Host
    • 57
    • 9
    • 3
    Mouse
    Clonality
    • 59
    • 9
    Monoclonal
    Conjugate
    • 30
    • 8
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-ABC antibody is un-conjugated
    Application
    • 53
    • 17
    • 17
    • 10
    • 10
    • 10
    • 8
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    No Cross-Reactivity
    Goat, Dog (Canine), Guinea Pig, Rabbit, Mouse (Murine), Chicken, Amphibian
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Macaque, Bovine, Baboon, Gorilla, Shrew.
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Purified human tonsil lymphocyte membranes
    Clone
    W6-32
    Isotype
    IgG2a
    Top Product
    Discover our top product HLA-ABC Primary Antibody
  • Application Notes
    ELISA. Immunoprecipitation. Immunohistochemistry on frozen sections. (Does not work on paraffin sections). Flow cytometry (1:50 - 1:100): use 10 μL of diluted antibody to label 1x10^6 cells or 100 μL whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, (2008) (PubMed).

    Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, (2005) (PubMed).

    Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).

  • Target
    HLA-ABC
    Alternative Name
    HLA Class I ABC (HLA-ABC Products)
    Synonyms
    HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody
    Background
    The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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