Doc 2a/b antibody

Catalog No. ABIN1742365

Product Details

Target: Doc 2a/b

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Detects preferentially Doc 2a

Cross-Reactivity (Details): some cross-reactivity to Doc 2b.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant full length rat Doc 2a protein.

Application Details

Application Notes: WB: 1 : 1000
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Schütz, Zilly, Lang, Jahn, Bruns: "A dual function for Munc-18 in exocytosis of PC12 cells." in: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, (2005) (PubMed).

Target Details

Target: Doc 2a/b