Vascular Adhes Adhesion Protein-1 antibody

Catalog No. ABIN2191843

Product Details

Target: Vascular Adhes Adhesion Protein-1

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Sterility: 0.2 μm filtered

Clone: 7-88

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user's experimental setting. Positive control Mouse VAP-1-transfected CHO cells (ref. 2) Negative control Mock-transfected CHO cells (Ref. 2)

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References

Iffiú-Soltész, Mercader, Daviaud, Boucher, Carpéné: "Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db-/- mice." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 118, Issue 7, pp. 1071-7, (2011) (PubMed).

Merinen, Irjala, Salmi, Jaakkola, Hänninen, Jalkanen: "Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse." in: The American journal of pathology, Vol. 166, Issue 3, pp. 793-800, (2005) (PubMed).

Bonder, Norman, Swain, Zbytnuik, Yamanouchi, Santamaria, Ajuebor, Salmi, Jalkanen, Kubes: "Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1." in: Immunity, Vol. 23, Issue 2, pp. 153-63, (2005) (PubMed).

Abella, García-Vicente, Viguerie, Ros-Baró, Camps, Palacín, Zorzano, Marti: "Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner." in: Diabetologia, Vol. 47, Issue 3, pp. 429-38, (2004) (PubMed).

Target Details

Target: Vascular Adhes Adhesion Protein-1

Background: The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein (VAP-1) which is a 7 88 Protein-1 glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane N terminal membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which VAP 1) presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 bound VAP belongs to enzymes called semicarbamizide semicarbamizide-sensitive amine oxidases, which contain copper as a , cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, primary aldehyde, and ammonia. VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells t the majority of VAP-1 is stored within intracellular granules and 1 intracel translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. infiltration Furthermore, the end end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct insulin cytotoxicity. In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been db VAP observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction obobserved in these diseases. Moreover, inhibition of VAP . VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth. s The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of 7 88 inflammation. Aliases Amine oxidase copper containing 3, AOC3, SSAO, membrane copper amine oxidase 3 Immunogen Vessels from mouse lymph nodes essels