MDM4-binding Protein antibody (pSer367)

Catalog No. ABIN3201009

Product Details anti-MDM4-binding Protein Antibody

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Binding Specificity: pSer367

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MDM4-binding Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Characteristics: Product: Mouse monoclonal antibody (clone #15) specific to the MdmX protein phosphorylated at Ser367, Human and mouse MdmX proteins phosphorylated at Ser367

Purification: Purified

Immunogen: A synthetic peptide corresponding to a sequence of human Mdmx protein surrounding pSer367

Clone: #15

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. ELISA
4. Indirect immuno-staining

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-MDM4-binding Protein antibody (ABIN3201009)

Chen, Gilkes, Pan, Lane, Chen: "ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage." in: The EMBO journal, Vol. 24, Issue 19, pp. 3411-22, (2005) (PubMed).

Okamoto, Kashima, Pereg, Ishida, Yamazaki, Nota, Teunisse, Migliorini, Kitabayashi, Marine, Prives, Shiloh, Jochemsen, Taya: "DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9608-20, (2005) (PubMed).

Target Details for MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Alternative Name: Mdmx/Hdmx (MDM4 Products)

Synonyms: mdmx antibody, HDMX antibody, MDMX antibody, MRP1 antibody, wu:fa09h09 antibody, wu:fi33d10 antibody, 4933417N07Rik antibody, AA414968 antibody, AL023055 antibody, AU018793 antibody, AU021806 antibody, C85810 antibody, Mdmx antibody, MDM4, p53 regulator S homeolog antibody, MDM4, p53 regulator antibody, transformed mouse 3T3 cell double minute 4 antibody, mdm4.S antibody, MDM4 antibody, Mdm4 antibody, mdm4 antibody

Background: MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80- kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.

UniProt: O15151

Pathways: Cell Division Cycle