TUBB antibody

Catalog No. ABIN93914

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Pig, Chicken, Tetrahymena, Fish, Paramecium, Nicotiana tabacum, Arabidopsis

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.

Cross-Reactivity (Details): Broad species reactivity

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Beta-subunits of porcine brain tubulin.

Clone: TU-06

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry: Recommended dilution: 2 μg/mL, fixed and permeabilized cells, positive control: 3T3 mouse embryonal fibroblast cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: heart.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TUBB antibody (ABIN93914)

Tobita, Liu, Janczewski, Tinney, Nonemaker, Augustine, Stolz, Shroff, Keller: "Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 4, pp. H1829-37, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Janisch, Hozák, Dráber: "Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex." in: Protoplasma, Vol. 225, Issue 3-4, pp. 157-67, (2005) (PubMed).

Solecki, Model, Gaetz, Kapoor, Hatten: "Par6alpha signaling controls glial-guided neuronal migration." in: Nature neuroscience, Vol. 7, Issue 11, pp. 1195-203, (2004) (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-tubulin (TUBB Products)

Background: Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB

Gene ID: 81027

UniProt: Q9H4B7

Pathways: Microtubule Dynamics, M Phase