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pan Keratin antibody

panKRT Reactivity: Mammalian FACS, WB, IHC (p), ICC, IP Host: Mouse Monoclonal C-11 unconjugated
Catalog No. ABIN94275
  • Target See all pan Keratin (panKRT) Antibodies
    pan Keratin (panKRT)
    Reactivity
    • 82
    • 58
    • 39
    • 34
    • 22
    • 20
    • 20
    • 19
    • 19
    • 15
    • 15
    • 14
    • 10
    • 5
    • 1
    • 1
    • 1
    Mammalian
    Host
    • 53
    • 34
    • 1
    Mouse
    Clonality
    • 56
    • 32
    Monoclonal
    Conjugate
    • 32
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This pan Keratin antibody is un-conjugated
    Application
    • 54
    • 53
    • 49
    • 24
    • 18
    • 16
    • 14
    • 14
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
    Cross-Reactivity (Details)
    Mammalian
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
    Clone
    C-11
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
    Immunohistochemistry: Recommended dilution: 2-8 μg/mL.
    Western blotting: Recommended dilution: 1-2 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Haun, Devaraj, Marinelli, Lee, Weissleder: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, (2011) (PubMed).

    Bianchi, Bombelli, Raimondo, Torsello, Angeloni, Ferrero, Di Stefano, Chinello, Cifola, Invernizzi, Brambilla, Magni, Pitto, Zanetti, Mocarelli, Perego: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, (2010) (PubMed).

    Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

    Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

    Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

    Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

  • Target
    pan Keratin (panKRT)
    Alternative Name
    Cytokeratin (Pan-reactive) (panKRT Products)
    Synonyms
    xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody
    Background
    Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT
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