DHL™ Cell Cytotoxicity Assay Kit

Catalog No. ABIN1882495

Product Details

Target: LDH

Detection Method: Fluorometric

Application: Enzyme Activity Assay (EAA)

Characteristics: The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components C and D can be stored at room temperature for convenience.

References

Choi, Lee, Na, An, Hyeon, Seo: "In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines." in: Bioprocess and biosystems engineering, Vol. 33, Issue 1, pp. 21-30, (2009) (PubMed).

Leach, Kilburn, Petkova, Romero, Armant: "Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor." in: American journal of obstetrics and gynecology, Vol. 198, Issue 4, pp. 471.e1-7; discussion 471.e7-8, (2008) (PubMed).

Wolff, Chiang, Smith, Romero, Armant: "Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells." in: Biology of reproduction, Vol. 77, Issue 1, pp. 53-60, (2007) (PubMed).

Armant, Kilburn, Petkova, Edwin, Duniec-Dmuchowski, Edwards, Romero, Leach: "Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor." in: Development (Cambridge, England), Vol. 133, Issue 4, pp. 751-9, (2006) (PubMed).

Target Details

Target: LDH

Background: The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity.