Mouse monoclonal antibody raised against native CD300A.
Specificity
This antibody reacts with CD300a, a 60 KDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.
Cross-Reactivity
Human
Immunogen
Native purified CD300A from human HPB acute lymphoid leukaemia cell line.
Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Dimasi, Roessle, Moran, Candiano, Svergun, Biassoni: "Molecular analysis and solution structure from small-angle X-ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a)." in: International journal of biological macromolecules, Vol. 40, Issue 3, pp. 193-200, (2007) (PubMed).
Alvarez, Tang, Coligan, Borrego: "The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling." in: Molecular immunology, Vol. 45, Issue 1, pp. 253-8, (2007) (PubMed).
Bachelet, Munitz, Levi-Schaffer: "Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a." in: The Journal of allergy and clinical immunology, Vol. 117, Issue 6, pp. 1314-20, (2006) (PubMed).