Asialoglycoprotein Receptor antibody
Reactivity: Rat
FACS, WB, IA
Host: Mouse
Monoclonal
8D7
unconjugated
(5 references)-
- Target
- Asialoglycoprotein Receptor
- Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunoassay (IA)
- Cross-Reactivity (Details)
- Cross reactivity: Human : Yes
- Sterility
- 0.2 μm filtered
- Clone
- 8D7
- Isotype
- IgG1
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- Application Notes
- For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Rat hepatocytes control Negative Rat kidney, pancreas, small intestine, colon control
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors." in: Molecular biology of the cell, Vol. 17, Issue 10, pp. 4300-17, (2006) (PubMed).
: "A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 26, Issue 1, pp. 55-60, (2003) (PubMed).
: "Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody." in: Acta medica Okayama, Vol. 56, Issue 3, pp. 135-9, (2002) (PubMed).
: "Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes." in: Liver, Vol. 7, Issue 5, pp. 251-9, (1988) (PubMed).
: "Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes." in: Gastroenterologia Japonica, Vol. 21, Issue 3, pp. 238-44, (1986) (PubMed).
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: "A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors." in: Molecular biology of the cell, Vol. 17, Issue 10, pp. 4300-17, (2006) (PubMed).
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- Target
- Asialoglycoprotein Receptor
- Background
- The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero- oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR. Aliases ASGPR 1, ASGP-R 1, Hepatic lectin 1, MHL-1 Immunogen Crude rat liver membrane extracts
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