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Clathrin antibody (AA 156-173)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio) IHC, WB, ICC Host: Mouse Monoclonal 57-4 unconjugated
Catalog No. ABIN1742245
  • Target See all Clathrin products
    Clathrin
    Binding Specificity
    AA 156-173
    Reactivity
    Human, Mouse, Rat, Zebrafish (Danio rerio)
    Host
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 2
    • 2
    Monoclonal
    Conjugate
    • 4
    This Clathrin antibody is un-conjugated
    Application
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
    Specificity
    Specific for both neuronal light chains
    Cross-Reactivity (Details)
    does not cross-react with the non-neuronal variants.
    Purification
    purified IgG
    Immunogen
    Synthetic peptide IADKAFYQQPDADTIGYV (aa 156-173) corresponding to the spliced insert of the neuronal light chain variants.
    Clone
    57-4
    Isotype
    IgG1
  • Application Notes
    WB: 1 : 5000 up to 1 : 10000 (AP staining)
    IP: not tested yet
    ICC: 1 : 1000
    IHC-P: 1 : 2000
    Comment

    Suitable for immunogold electron microscopy.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

    Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

    Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hellwig, Hack, Kowalski, Brunne, Jarowyj, Unger, Bock, Junghans, Frotscher: "Role for Reelin in neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).

    Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

    Krauss, Kukhtina, Pechstein, Haucke: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).

    Johnson, Brown, Pulliam, Anderson, Johnson: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." in: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).

    von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

    Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

    Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

  • Target
    Clathrin
    Abstract
    Clathrin Products
    Synonyms
    CG9012 antibody, CHC antibody, CLH antibody, Cla antibody, D-Chc antibody, Dmel\\CG9012 antibody, chc antibody, dCHC antibody, l(1)13Fb antibody, l(1)G0438 antibody, l(1)VI antibody, CG6948 antibody, CLC antibody, Dmel\\CG6948 antibody, clc antibody, Clathrin heavy chain antibody, Clathrin light chain antibody, Chc antibody, Clc antibody
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