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SMURF1 antibody (N-Term)

SMURF1 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB04101 unconjugated
Catalog No. ABIN388828
  • Target See all SMURF1 Antibodies
    SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
    Binding Specificity
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 66-96, N-Term
    Reactivity
    • 57
    • 22
    • 3
    Human, Mouse
    Host
    • 53
    • 4
    Rabbit
    Clonality
    • 54
    • 3
    Polyclonal
    Conjugate
    • 22
    • 7
    • 6
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SMURF1 antibody is un-conjugated
    Application
    • 47
    • 39
    • 28
    • 10
    • 4
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Predicted Reactivity
    X
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
    Clone
    RB04101
    Isotype
    Ig Fraction
    Top Product
    Discover our top product SMURF1 Primary Antibody
  • Application Notes
    WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:10~50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
    Expiry Date
    6 months
  • He, Yang, Isales, Shi: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-? inhibition of mesenchymal stem cell osteogenic differentiation." in: PLoS ONE, Vol. 7, Issue 3, pp. e31717, (2012) (PubMed).

    Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).

    Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).

    Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).

  • Target
    SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
    Alternative Name
    SMURF1 (SMURF1 Products)
    Synonyms
    4930431E10Rik antibody, mKIAA1625 antibody, RGD1309707 antibody, SMAD specific E3 ubiquitin protein ligase 1 antibody, E3 ubiquitin-protein ligase SMURF1 antibody, SMAD specific E3 ubiquitin protein ligase 1 L homeolog antibody, SMURF1 antibody, LOC100540915 antibody, smurf1 antibody, Smurf1 antibody, smurf1.L antibody
    Background
    Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
    Molecular Weight
    86114
    Gene ID
    57154
    NCBI Accession
    NP_001186776, NP_065162, NP_851994
    UniProt
    Q9HCE7
    Pathways
    Regulation of Cell Size
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