KCNC1 antibody (pSer503)

Catalog No. ABIN372706

Product Details anti-KCNC1 Antibody

Target: KCNC1 (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 1 (KCNC1))

Binding Specificity: pSer503

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Specificity: This antibody recognizes the ~100k KCNC1 (Kv3.1) protein phosphorylated at Ser503 in Rat brain extracts.

Cross-Reactivity (Details): Species reactivity (tested):Rat and Mouse.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser503 of KCNC1 conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Dot Blot: 1/1000. Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-KCNC1 antibody (ABIN372706)

Ahmed, Spikings, Zhou, Thompsett, Zhang: "Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin-streptavidin detection system." in: Journal of immunological methods, Vol. 406, pp. 143-7, (2014) (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

Chernova, Steinert, Guerin, Nicotera, Forsythe, Smith: "Neurite degeneration induced by heme deficiency mediated via inhibition of NMDA receptor-dependent extracellular signal-regulated kinase 1/2 activation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 32, pp. 8475-85, (2007) (PubMed).

Target Details for KCNC1

Target: KCNC1 (Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 1 (KCNC1))

Alternative Name: KCNC1 (KCNC1 Products)

Synonyms: kv3.1 antibody, C230009H10Rik antibody, KShIIIB antibody, KV4 antibody, Kcr2-1 antibody, Kv3.1 antibody, NGK2 antibody, Shaw antibody, Kv4 antibody, NGK2-KV4 antibody, KV3.1 antibody, KCNC2 antibody, zgc:194940 antibody, zgc:194950 antibody, potassium voltage-gated channel subfamily C member 1 antibody, potassium voltage-gated channel, Shaw-related subfamily, member 1 antibody, potassium voltage gated channel, Shaw-related subfamily, member 1 antibody, potassium voltage-gated channel, Shaw-related subfamily, member 1a antibody, KCNC1 antibody, kcnc1 antibody, Kcnc1 antibody, kcnc1a antibody

Background: Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995, Pongs, 1999, Blaine and Ribera, 1998, Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999). Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).Synonyms: Kv4, NGK2, Potassium voltage-gated channel subfamily C member 1, Voltage-gated potassium channel subunit Kv3.1

Gene ID: 25327

NCBI Accession: NP_036988

UniProt: P25122